Isolation of tissue-type plasminogen activator, cathepsin H, and non-specific cross-reacting antigen from SK-PC-1 pancreas cancer cells using subtractive hybridization

FEBS Lett. 1996 Apr 29;385(1-2):72-6. doi: 10.1016/0014-5793(96)00352-3.


We have used subtractive hybridization to isolate cDNAs overexpressed in SK-PC-1 pancreas cancer cells. Forty-five independent clones corresponding to 11 genes were identified. Their expression in cultured pancreas cancer cells, normal pancreas tissue, and normal exocrine pancreas cultures was examined by Northern blotting. cDNA clones can be grouped into two broad categories: (1) those corresponding to genes expressed at high levels both in tumor cell lines and in primary cultures of normal pancreas, but not in normal tissue (i.e. thymosin beta4(3), cytokeratin 18, beta-actin, pyruvate kinase and mitochondrial genes); and (2) those corresponding to genes expressed at high levels in pancreas cancer cultures but not in normal pancreas tissue or cultured cells (i.e. tissue-type plasminogen activator and cathepsin H). The overexpression of these proteases in pancreas cancers suggests that they play a role in the aggressive biological behavior of this tumor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / genetics*
  • Cathepsin H
  • Cathepsins / genetics*
  • Cell Adhesion Molecules*
  • Cloning, Molecular / methods
  • Cysteine Endopeptidases*
  • Cytoskeletal Proteins / genetics
  • DNA, Complementary / genetics
  • DNA, Neoplasm / genetics
  • Gene Expression
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Membrane Glycoproteins / genetics*
  • Nucleic Acid Hybridization
  • Pancreas / physiology
  • Pancreatic Neoplasms / chemistry
  • Pancreatic Neoplasms / genetics*
  • Pancreatic Neoplasms / immunology
  • RNA, Messenger / analysis
  • RNA, Neoplasm / analysis
  • Sequence Analysis, DNA
  • Tissue Plasminogen Activator / genetics*
  • Tumor Cells, Cultured


  • Antigens, Neoplasm
  • Cell Adhesion Molecules
  • Cytoskeletal Proteins
  • DNA, Complementary
  • DNA, Neoplasm
  • Membrane Glycoproteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Cathepsins
  • Tissue Plasminogen Activator
  • Cysteine Endopeptidases
  • CTSH protein, human
  • Cathepsin H