Cytoskeletal control of rectification and expression of four substates in cardiac inward rectifier K+ channels

FASEB J. 1996 Feb;10(2):357-61. doi: 10.1096/fasebj.10.2.8641571.

Abstract

Cardiac inward rectifiers may have a three-barrel channel structure, based on evidence for three substates in single-channel recordings. However, some reports indicate four substates, a feature more compatible with the four-subunit structure for which there is evidence in cloned voltage-activated K+ channels. Here we show that although the fourth is easily missed, inward rectifier channels have four substates whose expression is controlled by intracellular Ca(2+) ions. Fourth substate openings also appear after rectification loss in intracellular divalent caution-free solution. We find that this process is accelerated by cytochalasin, a microfilament disrupter. Cytochalasin also abolishes Ca(2+), but not Mg(2+),-induced rectification by restoring fourth substate openings. Thus, cytoskeletal elements control Ca(2+)-dependent substate expression and rectification in native inwardly rectifying K+ channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism
  • Cytoskeleton / physiology*
  • Guinea Pigs
  • In Vitro Techniques
  • Magnesium / pharmacology
  • Myocardium / metabolism*
  • Potassium Channels / physiology*

Substances

  • Potassium Channels
  • Magnesium
  • Calcium