Analysis of heterophilic cell adhesion mediated by CD66b and CD66c using their soluble recombinant proteins

Biochem Biophys Res Commun. 1996 Feb 27;219(3):842-7. doi: 10.1006/bbrc.1996.0320.

Abstract

The heterophilic cell adhesion mediated by CD66b (carcinoembryonic antigen (CEA) gene family member 6, CGM6) and CD66c (nonspecific cross-reacting antigen, NCA), both CEA family members expressed on neutrophils, was investigated using their soluble recombinant proteins prepared in silkworm larvae. The recombinant CD66b and CD66c immobilized on plastic bound CHO transfectants expressing CD66c and CD66b, respectively. Their deglycosylated forms retained the adhesion activity, suggesting that their carbohydrate portions are not prerequisite for the binding. This cell adhesion appeared to be mediated via interaction between the N domains of CD66b and CD66c, because CD66 antibodies recognizing their N domains inhibited the binding. Neutrophils, when activated, adhered to the immobilized CD66b and CD66c. In addition, the binding of primed neutrophils to the antigens induced superoxide anion release. The cell adhesion mediated by CD66b and CD66c may play a role in interaction between neutrophils or between neutrophils and epithelial cells expressing CD66c in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD
  • Antigens, Neoplasm*
  • Bombyx / metabolism
  • CHO Cells
  • Calcimycin / pharmacology
  • Carcinoembryonic Antigen / biosynthesis
  • Carcinoembryonic Antigen / genetics
  • Cell Adhesion Molecules / physiology*
  • Cell Adhesion*
  • Cell Line
  • Colonic Neoplasms
  • Cricetinae
  • GPI-Linked Proteins
  • Humans
  • Larva
  • Membrane Glycoproteins / biosynthesis
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / physiology*
  • Multigene Family
  • Neutrophils / drug effects
  • Neutrophils / physiology
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Superoxides / blood
  • Transfection

Substances

  • Antigens, CD
  • Antigens, Neoplasm
  • CEACAM6 protein, human
  • CEACAM8 protein, human
  • Carcinoembryonic Antigen
  • Cell Adhesion Molecules
  • GPI-Linked Proteins
  • Membrane Glycoproteins
  • Recombinant Proteins
  • Superoxides
  • Calcimycin