Interleukin-6 enhances glucose transport in 3T3-L1 adipocytes

Biochem Biophys Res Commun. 1996 Mar 18;220(2):241-5. doi: 10.1006/bbrc.1996.0389.


To study the effect of interleukin-6 (IL-6) on glucose transport, 2-deoxy-D-glucose (2-DOG) uptake in 3T3-L1 adipocytes after incubation with IL-6 was measured. IL-6 increased 2-DOG uptake maximally after 5 hours by 30 +/- 12%, with a half-maximal effect at an IL-6 concentration of approximately 800 U/mL. 3-O-methylglucose uptake was stimulated in a similar way, indicating that IL-6 enhanced glucose transport rather than phosphorylation. IL-6-induced glucose transport was additive to the effect of insulin. Addition of cycloheximide did not affect the stimulatory action of IL-6, although cycloheximide itself had profound effects on basal glucose transport. We conclude that IL-6 may enhance glucose uptake by increasing GLUT-1 intrinsic activity.

MeSH terms

  • 3-O-Methylglucose
  • 3T3 Cells
  • Adipocytes / metabolism*
  • Animals
  • Biological Transport / drug effects
  • Cycloheximide / pharmacology
  • Deoxyglucose / metabolism
  • Glucose / metabolism*
  • Insulin / pharmacology
  • Interleukin-6 / pharmacology*
  • Kinetics
  • Methylglucosides / metabolism
  • Mice
  • Phosphorylation
  • Recombinant Proteins / pharmacology


  • Insulin
  • Interleukin-6
  • Methylglucosides
  • Recombinant Proteins
  • 3-O-Methylglucose
  • Cycloheximide
  • Deoxyglucose
  • Glucose