A new plasmid-encoded proteic killer gene system: cloning, sequencing, and analyzing hig locus of plasmid Rts1

Biochem Biophys Res Commun. 1996 Mar 18;220(2):280-4. doi: 10.1006/bbrc.1996.0396.

Abstract

A new proteic killer gene system, hig, was identified on the plasmid Rts1. The hig locus consisting of a higA and higB is directly related to the temperature sensitive host cell growth conferred by Rts1. We proved that higB encoding presumably a 92-amino-acid polypeptide inhibited segregation of plasmid free cells, and higA encoding a 104-amino-acid polypeptide suppressed the higB function both in cis and in trans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Cloning, Molecular*
  • Escherichia coli / genetics
  • Escherichia coli Proteins*
  • Molecular Sequence Data
  • Phenotype
  • Plasmids*
  • Polymerase Chain Reaction
  • Recombinant Proteins
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Temperature

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • HigA protein, E coli
  • HigB protein, E coli
  • Recombinant Proteins

Associated data

  • GENBANK/U43847