Purification and identification of allergenic alpha (2u)-globulin species of rat urine

Biochim Biophys Acta. 1996 Jun 4;1290(2):129-34. doi: 10.1016/0304-4165(96)00006-2.


Amino-acid compositional and sequence analyses as well as mass spectrometric determinations of purified rat urine proteins, previously termed prealbumin and alpha(2)-euglobulin, have revealed a high homology between the two forms which have now been identified as alpha(2)-globulin species. The "prealbumin' fraction was found to correspond to alpha(2u)-globulin originating from salivary gland and the 'alpha(2)-euglobulin' fraction was identical with the major urinary protein (MUP) or alpha(2u)-globulin. The results indicate that the two major protein fractions of rat urine constitute different forms of the same parent protein, alpha(2u)-globulin, having no amino-acid sequence resemblance to prealbumin (transthyretin) of rat serum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / chemistry
  • Allergens / isolation & purification*
  • Allergens / urine
  • Alpha-Globulins / chemistry
  • Alpha-Globulins / immunology
  • Alpha-Globulins / urine*
  • Amino Acid Sequence
  • Animals
  • Male
  • Molecular Sequence Data
  • Molecular Weight
  • Proteins / chemistry
  • Proteins / immunology
  • Proteins / isolation & purification*
  • Rats
  • Rats, Sprague-Dawley


  • Allergens
  • Alpha-Globulins
  • Proteins
  • alpha 2u globulin
  • major urinary proteins