Use of the polymerase chain reaction and DNA sequencing for detection of Bartonella quintana in the aortic valve of a patient with culture-negative infective endocarditis

Clin Infect Dis. 1995 Oct;21(4):891-6. doi: 10.1093/clinids/21.4.891.


We used the polymerase chain reaction (PCR) and broad-range bacterial primers, combined with DNA sequencing, to identify Bartonella quintana as the etiologic agent in a case of culture-negative infective endocarditis; all blood cultures, as well as the bacterial cultures of the resected aortic valve and vegetations, remained negative. PCR was used to amplify bacterial 16S rDNA from a template prepared from the aortic valve vegetation. The amplified 16S rDNA produced a nucleotide sequence that was 99.79% identical to the B. quintana rDNA sequence. The patient had a highly elevated level of serum antibodies to Bartonella antigen (1:8,192).

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Bacterial / blood
  • Aortic Valve / microbiology*
  • Bartonella quintana / genetics
  • Bartonella quintana / immunology
  • Bartonella quintana / isolation & purification*
  • Base Sequence
  • DNA Primers
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Endocarditis, Bacterial / diagnosis*
  • Follow-Up Studies
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA


  • Antibodies, Bacterial
  • DNA Primers
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/U28268