Abstract
Mutations in the Drosophila gene dreadlocks (dock) disrupt photoreceptor cell (R cell) axon guidance and targeting. Genetic mosaic analysis and cell-type-specific expression of dock transgenes demonstrate dock is required in R cells for proper innervation. Dock protein contains one SH2 and three SH3 domains, implicating it in tyrosine kinase signaling, and is highly related to the human proto-oncogene Nck. Dock expression is detected in R cell growth cones in the target region. We propose Dock transmits signals in the growth cone in response to guidance and targeting cues. These findings provide an important step for dissection of signaling pathways regulating growth cone motility.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adaptor Proteins, Signal Transducing
-
Amino Acid Sequence
-
Animals
-
Axons / metabolism
-
Drosophila / genetics
-
Drosophila / growth & development
-
Drosophila / metabolism*
-
Drosophila Proteins
-
Female
-
Humans
-
Male
-
Molecular Sequence Data
-
Mosaicism
-
Mutation
-
Nerve Tissue Proteins / genetics
-
Nerve Tissue Proteins / metabolism*
-
Oncogene Proteins / genetics
-
Oncogene Proteins / metabolism
-
Photoreceptor Cells, Invertebrate / growth & development
-
Photoreceptor Cells, Invertebrate / metabolism*
-
Protein-Tyrosine Kinases / metabolism
-
Sequence Homology, Amino Acid
-
Signal Transduction
-
src Homology Domains
Substances
-
Adaptor Proteins, Signal Transducing
-
Drosophila Proteins
-
Nck protein
-
Nerve Tissue Proteins
-
Oncogene Proteins
-
dock protein, Drosophila
-
Protein-Tyrosine Kinases