Flow cytometry analysis of alpha1-adrenoceptor subtypes

FEBS Lett. 1996 May 20;386(2-3):141-8. doi: 10.1016/0014-5793(96)00388-2.


To characterize the alpha1-adrenoceptor subtypes, we developed a flow cytometry method using the fluorescent ligand BODIPY-FL prazosin and the anti-peptide antibody against the alpha1b-adrenoceptor amino terminus (designated 1B-N1-C) as probes. Three alpha1-adrenoceptors (alpha1a, alpha1b and alpha1d) expressed in CHO cells were detected by BODIPY-FL prazosin; however, only alpha1b-adrenoceptor subtype was detected by the anti-peptide antibody 1B-N1-C. Furthermore, the flow cytometry analysis with 1B-N1-C specifically identified alpha1b-adrenoceptor in native cells of hamster DDT1-MF2 cells, rat hepatocytes and cardiomyocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Boron Compounds / chemistry
  • CHO Cells
  • Cells, Cultured
  • Chlorocebus aethiops
  • Cricetinae
  • Flow Cytometry / methods*
  • Fluorescent Dyes / chemistry
  • Humans
  • Liver / cytology
  • Molecular Sequence Data
  • Molecular Structure
  • Myocardium / cytology
  • Rabbits
  • Rats
  • Receptors, Adrenergic, alpha-1 / analysis*
  • Receptors, Adrenergic, alpha-1 / classification


  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • ADRA1A protein, human
  • ADRA1B protein, human
  • ADRA1D protein, human
  • Adra1a protein, rat
  • Adra1b protein, rat
  • Adra1d protein, rat
  • Boron Compounds
  • Fluorescent Dyes
  • Receptors, Adrenergic, alpha-1