Localization of the nic-7, ac-29 and thi-10 genes within the mating-type locus of Chlamydomonas reinhardtii

Genetics. 1995 Oct;141(2):543-9. doi: 10.1093/genetics/141.2.543.

Abstract

The tight linkage observed between the mating-type (mt) locus of Chlamydomonas reinhardtii and three auxotrophic mutations--nic-7 (nicotinamide-requiring), ac-29 (acetate-requiring), and thi-10 (thiamine-requiring)--has led to the hypothesis that recombination is suppressed in the mt region. The physical location of these three genes has been established by transformation with sets of cloned DNA from the mt region. They lie to the left and right of the highly rearranged (R) domain of the mt locus, which has been proposed to be responsible for teh recombinational suppression in the region. The cloned nic-7+ and thi-10+ genes will be useful as selectable markers for cotransformation markers for cotransformation experiments.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Chlamydomonas reinhardtii / genetics*
  • Chlamydomonas reinhardtii / physiology
  • Chromosome Mapping*
  • Crosses, Genetic
  • DNA, Fungal / metabolism
  • DNA, Protozoan / metabolism
  • Genes, Plant
  • Genes, Protozoan
  • Genetic Linkage
  • Methylation
  • Recombination, Genetic*
  • Restriction Mapping
  • Species Specificity
  • Transformation, Genetic

Substances

  • DNA, Fungal
  • DNA, Protozoan