Cell-cycle-dependent expression of the STK-1 gene encoding a novel murine putative protein kinase

Gene. 1996 Mar 9;169(2):197-201. doi: 10.1016/0378-1119(95)00809-8.

Abstract

We have cloned a novel putative serine/threonine kinase-encoding gene, designed STK-1, from murine embryonic stem (ES) cell and testis cDNA libraries. The kinase most closely related to STK-1 is Xenopus laevis XLP46 protein kinase which shows 71% amino-acid identity to STK-1 between their kinase domains. Nevertheless, STK-1 is conserved throughout phylogeny with hybridizing sequences being detected in DNA from mammals, amphibians, insects and yeast. STK-1 mRNA is detected in testis, intestine and spleen, tissues that contain a large number of proliferating cells, but not in other tissues. All cell lines tested expressed STK-1 mRNA with levels being dependent upon proliferation rates. In NIH 3T3 cells, STK-1 is expressed in a cell-cycle-dependent fashion. These findings suggest a role for STK-1 in cell growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Aurora Kinase B
  • Aurora Kinases
  • Base Sequence
  • Cell Cycle*
  • Cell Division
  • Cell Line
  • Conserved Sequence
  • DNA, Complementary
  • Gene Expression / genetics*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics*
  • Sequence Homology, Nucleic Acid
  • Testis / physiology

Substances

  • DNA, Complementary
  • Aurkb protein, mouse
  • Aurora Kinase B
  • Aurora Kinases
  • Protein-Serine-Threonine Kinases

Associated data

  • GENBANK/D21099