BRO1, a novel gene that interacts with components of the Pkc1p-mitogen-activated protein kinase pathway in Saccharomyces cerevisiae

Mol Cell Biol. 1996 Jun;16(6):2585-93. doi: 10.1128/MCB.16.6.2585.

Abstract

Yeast cells with mutations in BRO1 display phenotypes similar to those caused by deletion of BCK1, a gene encoding a MEK kinase that functions in a mitogen-activated protein kinase pathway mediating maintenance of cell integrity. bro1 cells exhibit a temperature-sensitive growth defect that is suppressed by the addition of osmotic stabilizers or Ca2+ to the growth medium or by additional copies of the BCK1 gene. At permissive temperatures, bro1 mutants are sensitive to caffeine and respond abnormally to nutrient limitation. A null mutation in BRO1 is synthetically lethal with null mutations in BCK1, MPK1, which encodes a mitogen-activated protein kinase that functions downstream of Bck1p, or PKC1, a gene encoding a protein kinase C homolog that activates Bck1p. Analysis of the isolated BRO1 gene revealed that it encodes a novel, 97-kDa polypeptide which contains a putative SH3 domain-binding motif and is homologous to a protein of unknown function in Caenorhabditis elegans.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Caenorhabditis elegans / genetics
  • Calcium / pharmacology
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • Cell Division / drug effects
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • Genes, Fungal*
  • Helminth Proteins / genetics
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Protein Kinase C / genetics
  • Protein Kinase C / metabolism*
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Sequence Homology, Amino Acid

Substances

  • DNA, Fungal
  • Helminth Proteins
  • Protein Kinase C
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Calcium

Associated data

  • GENBANK/U37364