Identification of a human LIM-Hox gene, hLH-2, aberrantly expressed in chronic myelogenous leukaemia and located on 9q33-34.1

Oncogene. 1996 Mar 21;12(6):1205-12.


We describe the isolation of human LH-2, a putative transcription factor containing two cysteine-rich regions (LIM domains) and a homeobox (Hox) DNA-binding domain. High levels of hLH-2 expression were observed in all cases of chronic myelogenous leukaemia (CML) tested, regardless of disease status. hLH-2 was mapped to chromosome 9Q33-34.1, in the same region as the reciprocal translocation that creates the BCR-ABL chimera of the Philadelphia chromosome (Ph'), the hallmark of CML; hLH-2 was retained on the derivative 9 chromosome and is therefore centromeric of c-ABL. The proximity of hLH-2 to the breakpoint on chromosome 9 raises the possibility of cis-activation by the t(9;22)(q34;q11) translocation. In addition to finding hLH-2 expression in all cases of CML, expression was observed in lymphoid malignancies and myeloid cell lines, but not in primary cases of acute myelogenous leukaemia. The role of hLH-2 in the development or progression of leukaemia is not known. However, hLH-2 may prove useful as a marker of CML for monitoring residual disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • CHO Cells
  • Chromosome Mapping
  • Chromosomes, Human, Pair 22
  • Chromosomes, Human, Pair 9*
  • Cricetinae
  • DNA / genetics
  • DNA / isolation & purification
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / isolation & purification
  • Gene Expression
  • Genes, Homeobox*
  • Homeodomain Proteins / biosynthesis*
  • Homeodomain Proteins / genetics*
  • Humans
  • LIM-Homeodomain Proteins
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
  • Lymphocytes / metabolism
  • Lymphocytes / physiology
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Rats
  • Sheep
  • Transcription Factors / biosynthesis*
  • Transcription Factors / genetics*
  • Transcription, Genetic
  • Translocation, Genetic


  • DNA, Neoplasm
  • Homeodomain Proteins
  • LHX2 protein, human
  • LIM-Homeodomain Proteins
  • LIM-homeodomain protein LH-2
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/U11701