Abstract
The T cytoplasm of maize serves as a model for the nuclear restoration of cytoplasmic male sterility. The rf2 gene, one of two nuclear genes required for fertility restoration in male-sterile T-cytoplasm (cmsT) maize, was cloned. The protein predicted by the rf2 sequence is a putative aldehyde dehydrogenase, which suggests several mechanisms that might explain Rf2-mediated fertility restoration in cmsT maize. Aldehyde dehydrogenase may be involved in the detoxification of acetaldehyde produced by ethanolic fermentation during pollen development, may play a role in energy metabolism, or may interact with URF13, the mitochondrial protein associated with male sterility in cmsT maize.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acetaldehyde / metabolism
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Aldehyde Dehydrogenase / chemistry
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Aldehyde Dehydrogenase / genetics*
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Aldehyde Dehydrogenase / metabolism
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Alleles
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Amino Acid Sequence
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Cell Nucleus
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Cloning, Molecular
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Crosses, Genetic
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Cytoplasm / genetics
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Cytoplasm / physiology
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Energy Metabolism
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Genes, Plant*
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Intracellular Membranes / metabolism
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Mitochondria / genetics
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Mitochondria / metabolism
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Mitochondrial Proteins*
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Molecular Sequence Data
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Pollen / physiology
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Zea mays / genetics*
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Zea mays / physiology*
Substances
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Mitochondrial Proteins
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Nuclear Proteins
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Plant Proteins
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URF13 protein, Zea mays
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Aldehyde Dehydrogenase
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Acetaldehyde
Associated data
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GENBANK/P05091
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GENBANK/P20000
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GENBANK/U43082