Cloning and sequencing of the gene for Na+/H+ antiporter of Vibrio parahaemolyticus

Biochem Biophys Res Commun. 1996 May 24;222(3):774-9. doi: 10.1006/bbrc.1996.0820.

Abstract

A gene encoding an Na+/H+ antiporter was cloned from vibrio parahaemolyticus into the plasmid pBR322 and expressed in Escherichia coli cells. The gene enabled mutant E. coli cells to grow in the presence of 0.2 M NaCl (or 10 mM LiCl). These cells were originally unable to grow under such conditions because of the lack of major Na+(Li+)/H+ antiporters. We detected Na+/H+ antiporter activity due to the gene in membrane vesicles. The gene was sequenced and the deduced amino acid sequence was found to be 72% identical to the NhaB Na+/H+ antiporter of E. coli.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Genes, Bacterial
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sodium-Hydrogen Exchangers / genetics*
  • Sodium-Hydrogen Exchangers / metabolism
  • Vibrio parahaemolyticus / genetics*

Substances

  • DNA, Bacterial
  • Sodium-Hydrogen Exchangers