Expression of a novel member of estrogen response element-binding nuclear receptors is restricted to the early stages of chorion formation during mouse embryogenesis

Mech Dev. 1996 Feb;54(2):211-23. doi: 10.1016/0925-4773(95)00479-3.


Members of the nuclear hormone receptor gene family of transcription factors have been shown to be expressed in characteristic patterns during mouse organogenesis and postnatal development. Using an RT-PCR based screening assay, we have identified nuclear receptors expressed in embryonal carcinoma stem cells. One of the cDNAs characterized, mERR-2, was found to be expressed exclusively during a narrow developmental window in trophoblast progenitor cells between days 6.5 and 7.5 post coitum (p.c.). From 8.5 days p.c. and onwards, the mERR-2 gene activity evaded detection as analysed by in situ hybridization. We also show that the mERR-2 gene product and the estrogen receptor share a common target DNA-sequence recognition specificity unique among members of the gene family. Furthermore, efficient homodimerization and DNA-binding of the orphan receptor mERR-2 was found to be dependent on interaction with the heat shock protein 90, a molecular chaperone hitherto recognized to interact only with the steroid hormone receptor subgroup of nuclear receptors. Based on our results we suggest that the mouse orphan receptor mERR-2 has the potential to regulate overlapping gene networks with the estrogen receptor and may participate in signal transduction pathways during a short developmental period coinciding with the formation of the chorion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Differentiation
  • Cell Line
  • Chorion / embryology*
  • DNA / metabolism
  • DNA, Complementary
  • Embryonic and Fetal Development / genetics*
  • Female
  • Heat-Shock Proteins / metabolism
  • Male
  • Mice
  • Molecular Sequence Data
  • Protein Binding
  • RNA, Messenger / genetics
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Receptors, Estrogen / genetics*
  • Receptors, Estrogen / metabolism


  • DNA, Complementary
  • Esrrb protein, mouse
  • Heat-Shock Proteins
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Estrogen
  • DNA

Associated data

  • GENBANK/S82458