Bumetanide is not transported by the Ntcp or by the oatp: evidence for a third organic anion transporter in rat liver cells

Biochim Biophys Acta. 1996 Apr 19;1300(2):114-8. doi: 10.1016/0005-2760(95)00239-1.


The loop diuretic bumetanide which inhibits hepatic bile acid uptake competitively according to its transport kinetics has been proposed to serve as a substrate of a multispecific bile acid transport system in liver parenchymal cells. However, when the in vitro transcripts of two cloned hepatic bile acid uptake carriers, the Ntcp (Na+/taurocholate cotransporting polypeptide) and the oatp (organic anion transporting polypeptide), was expressed for three days in Xenopus laevis oocytes [3H]bumetanide uptake was not increased although bile acid uptake was stimulated. The data presented show that bumetanide is taken up by a third organic anion transport system which is different from the cloned bile acid transporters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Animals
  • Anion Transport Proteins
  • Biological Transport
  • Bumetanide / metabolism*
  • Carrier Proteins / metabolism*
  • Diuretics / metabolism*
  • Glycocholic Acid / metabolism
  • Ion Transport
  • Liver / metabolism*
  • Oocytes / metabolism
  • RNA, Antisense / metabolism
  • RNA, Antisense / pharmacology
  • RNA, Messenger / metabolism
  • Rats
  • Sodium / pharmacology
  • Xenopus laevis


  • ATP-Binding Cassette Transporters
  • Anion Transport Proteins
  • Carrier Proteins
  • Diuretics
  • RNA, Antisense
  • RNA, Messenger
  • taurocholate carrier protein, rat
  • Bumetanide
  • Sodium
  • Glycocholic Acid