Cytokines and programmed cell death in burkitt lymphoma cells

Biochem Mol Biol Int. 1995 Sep;37(1):17-24.

Abstract

Tumour necrosis Factor (TNF), Interleukin 1alpha and beta (IL-alpha and IL-beta), Interleukin 7 (IL-7) and Stem Cell Factor (SCF) are cytokines synthesized by immune system cells under stimulation of various agents. Apoptosis, or programmed cell death, is a process that appears in response to specific stimuli, apparently following an intrinsic program. In this work we examined, in RA-1 human lymphoblastoid B cell line, the effect induced by different cytokines in cell proliferation and in programmed cell death. After 48 hours of treatment is present an antiproliferative affects, detected by 3H-thymidine incorporation and morphological changes related to apoptotic process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects*
  • Burkitt Lymphoma / drug therapy
  • Burkitt Lymphoma / genetics
  • Burkitt Lymphoma / pathology*
  • Cell Differentiation / drug effects
  • Chromatin / drug effects
  • Cytokines / pharmacology*
  • Electrophoresis
  • Flow Cytometry
  • Humans
  • Interleukin-1 / pharmacology
  • Interleukin-7 / pharmacology
  • Recombinant Proteins / pharmacology
  • Stem Cell Factor / pharmacology
  • Thymidine / metabolism
  • Thymidine / pharmacokinetics
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Chromatin
  • Cytokines
  • Interleukin-1
  • Interleukin-7
  • Recombinant Proteins
  • Stem Cell Factor
  • Tumor Necrosis Factor-alpha
  • Thymidine