CLA1, a novel gene required for chloroplast development, is highly conserved in evolution

Plant J. 1996 May;9(5):649-58. doi: 10.1046/j.1365-313x.1996.9050649.x.


An albino mutant designated cla1-1 (for "cloroplastos alterados', or "altered chloroplasts') has been isolated from a T-DNA-generated library of Arabidopsis thaliana. In cla1-1 plants, chloroplast development is arrested at an early stage. cla1-1 plants behave like wild-type in their capacity to etiolate and produce anthocyanins indicating that the light signal transduction pathway seems to be unaffected. Genetic and molecular analyses show that the disruption of a single gene, CLA1, by the T-DNA insertion is responsible for the mutant phenotype. RNA expression patterns indicate that CLA1 is positively regulated by light and that it has different effects on the steady-state RNA levels of some nuclear- and chloroplast-encoded photosynthetic genes. Although the specific function of the CLA1 gene is still unknown, it encodes a novel protein conserved in evolution between photosynthetic bacteria and plants which is essential for chloroplast development in Arabidopsis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / classification
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins*
  • Base Sequence
  • Biological Evolution*
  • Carotenoids / biosynthesis
  • Chlorophyll / biosynthesis
  • Chloroplasts / physiology
  • Cloning, Molecular
  • Conserved Sequence*
  • DNA, Complementary
  • Gene Library
  • Genetic Complementation Test
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Open Reading Frames
  • Plant Proteins / biosynthesis*
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Restriction Mapping
  • Rhodobacter capsulatus / genetics
  • Sequence Homology, Amino Acid


  • Arabidopsis Proteins
  • CLA1 protein, Arabidopsis
  • DNA, Complementary
  • Plant Proteins
  • Recombinant Proteins
  • Chlorophyll
  • Carotenoids

Associated data

  • GENBANK/U27099