The homeotic target gene centrosomin encodes an essential centrosomal component

Cell. 1996 May 17;85(4):585-96. doi: 10.1016/s0092-8674(00)81258-1.

Abstract

The centrosomin (cnn) gene encodes a protein associated with mitotic centrosomes in Drosophila melanogaster and is a target of homeotic gene regulation. Here we report that CNN is an essential component of the centrosome. Loss of zygotic cnn expression disrupts the development of the second midgut constriction, the gastric caeca, and the nervous system. Embryos that lack maternal as well as zygotic cnn expression display defects in nuclear division, chromosome alignment, and microtubule organization, while adult flies that are mosaic for cnn-cells exhibit defects indicative of a block in cell proliferation. We propose that cnn provides an example of homeotic genes directly regulating the accumulation of essential cellular proteins to carry out segment-specific morphogenetic functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastoderm / physiology
  • Cell Cycle / physiology
  • Cell Nucleus / genetics
  • Centrosome / chemistry
  • Centrosome / physiology*
  • Clone Cells / physiology
  • Cytoplasm / chemistry
  • Drosophila / embryology
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Female
  • Gene Expression Regulation, Developmental / physiology
  • Germ-Line Mutation / physiology
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism
  • Leucine Zippers / genetics
  • Male
  • Microtubules / chemistry
  • Morphogenesis / genetics
  • Mutagenesis / physiology
  • Oogenesis / physiology
  • Phosphorylation
  • Spermatogenesis / physiology

Substances

  • Drosophila Proteins
  • Homeodomain Proteins
  • cnn protein, Drosophila