Characterization of the NHA1 gene encoding a Na+/H+-antiporter of the yeast Saccharomyces cerevisiae

FEBS Lett. 1996 May 27;387(1):89-93. doi: 10.1016/0014-5793(96)00470-x.


The NHA1 gene (2958 nt) encoding a putative Na(+)/H(+) antiporter (986 aa) in Saccharomyces cerevisiae was cloned by selection based on increased NaCl tolerance. The putative protein is highly similar to sodium/proton antiporters from Schizosaccharomyces pombe (gene sod2), and Zygosaccharomyces rouxii (gene Z-SOD2). Overexpression of the NHA1 gene results in higher and partially pH-dependent tolerance to sodium and lithium; its disruption leads to an increased sensitivity towards these ions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cation Transport Proteins*
  • Cloning, Molecular
  • DNA, Fungal
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Plasmids
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Sodium Chloride / pharmacology
  • Sodium-Hydrogen Exchangers / genetics
  • Sodium-Hydrogen Exchangers / metabolism*


  • Cation Transport Proteins
  • DNA, Fungal
  • Membrane Proteins
  • NHA1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sodium-Hydrogen Exchangers
  • Sodium Chloride

Associated data

  • GENBANK/X91258