Heat-labile uracil-DNA glycosylase: purification and characterization

FEBS Lett. 1996 Jun 10;388(1):1-4. doi: 10.1016/0014-5793(96)00444-9.


A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40 degrees C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli.

MeSH terms

  • Bacteria / enzymology
  • Base Sequence
  • DNA Glycosylases*
  • Enzyme Stability
  • Half-Life
  • Hot Temperature
  • Molecular Sequence Data
  • Molecular Weight
  • N-Glycosyl Hydrolases / chemistry
  • N-Glycosyl Hydrolases / isolation & purification*
  • N-Glycosyl Hydrolases / metabolism*
  • Uracil-DNA Glycosidase


  • DNA Glycosylases
  • N-Glycosyl Hydrolases
  • Uracil-DNA Glycosidase