Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type

Mol Gen Genet. 1977 Feb 28;151(1):77-81. doi: 10.1007/BF00446915.

Abstract

Protoplasts of methionine- and lysine-requiring h- mutants isolated from the L972 h- strain of Schizosaccharomyces pombe were fused. The protoplasts were obtained from the cells with enzymes produced by Trichoderma viride. When a mixture of the protoplasts was treated with 30% PEG 4000 solution containing 10 mM CaCl2, cell fusion and complementation was attained with a frequency of 0.17%. Both fusion partners were recovered among the spores after crossing of the fusion products with the strain M210 ade6 h+. Cytological and haploidization examinations showed that the fusion cells are not heterokaryons, and that the increased amount of genetic material is situated in one nucleus.

MeSH terms

  • Ascomycota*
  • Cell Fusion*
  • Conjugation, Genetic
  • F Factor
  • Methionine / metabolism
  • Mutation
  • Phenotype*
  • Protoplasts*
  • Schizosaccharomyces*
  • Tyrosine / metabolism

Substances

  • Tyrosine
  • Methionine