Sequence of the mouse adenovirus type-1 DNA encoding the 100-kDa, 33-kDa and DNA-binding proteins

Gene. 1996 Feb 12;168(2):183-7. doi: 10.1016/0378-1119(95)00715-6.

Abstract

The genomic nucleotide sequence for the region of 66 to 77 map units (m.u.) of mouse adenovirus type 1 (MAV-1) was determined and predicted to encode proteins homologous to the human adenovirus (Ad) 100-kDa, 33kDa and DNA-binding proteins (DBP). The putative MAV-1 100-kDa protein has 65-70% amino-acid similarity to 100-kDa proteins from five different human Ad serotypes. The mRNA for the putative 33-kDa protein is internally spliced within the coding sequence, as are its human Ad counterparts [Oosterom-Dragon and Anderson, J. Virol 45 (1983) 251-263]. The N-terminal region of the putative MAV-1 33-kDa protein has 41-44% similarity to two human Ad 33-kDa N-termini, and the C-terminal regions are more conserved, with 60-65% similarity. The MAV-1 DBP is predicted to be encoded in this region and was compared to six different human Ad DBP N-and C-termini. The N-termini of the MAV-1 and Ad DBP were 33-48% similar and the C-termini were 56-60% similar. The MAV-1 DBP contains conserved regions (CR) 1,2 and 3, and it retains important residues for a putative zinc finger (Zf) motif identified in Ad DBP [Eagle and Klessig, Virology 187 (1992) 777-787]. Additional sequence features of these proteins have also been identified.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics*
  • Adenoviridae / metabolism
  • Adenoviruses, Human / genetics
  • Adenoviruses, Human / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Conserved Sequence
  • DNA, Viral / chemistry*
  • DNA, Viral / metabolism
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / genetics
  • Humans
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Plasmids
  • Sequence Deletion
  • Sequence Homology, Amino Acid

Substances

  • DNA, Viral
  • DNA-Binding Proteins

Associated data

  • GENBANK/U23770