Sequence and Expression of a Bovine herpesvirus-1 Gene Homologous to the Glycoprotein K-encoding Gene of Herpes Simplex virus-1

Gene. 1996 Feb 12;168(2):189-93. doi: 10.1016/0378-1119(95)00776-8.

Abstract

In the bovine herpes virus-1 (BHV-1) genome, a gene equivalent to the glycoprotein k (gK)-encoding gene of other herpesviruses was identified and sequenced. The primary translation product is predicted to comprise 338 amino acids (aa) and to exhibit a molecular mass of 37.5 kDa. It possesses characteristics typical for membrane glycoproteins including a potential cleavable signal sequence, three transmembrane domains and two potential N-linked glycosylation sites. Comparison to the gK proteins of the other herpesviruses revealed aa sequence homologies of 46, 44, 53, 43, and 46% with the gK counterparts of herpes simplex viruses-1 and 2 (HSV-1 and 2), equine herpesvirus-1 (EHV-1), Marek's disease virus (MDV) and varicella zoster virus (VZV), respectively. A 30-kDa primary translation product was identified following in vitro translation of in vitro transcribed mRNA. When canine microsomal membranes were added to the translation reaction, a 38-kDa glycosylated protein was detected. Treatment with endoglycosidase F or H (endo or H) removed the glycosyl groups and reduced the apparent molecular mass of the 38-kDa glycoprotein.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Gene Expression*
  • Genes, Viral*
  • Genome, Viral
  • Glycosylation
  • Herpesviridae / genetics
  • Herpesvirus 1, Bovine / genetics*
  • Herpesvirus 1, Bovine / metabolism
  • Herpesvirus 1, Equid / genetics
  • Herpesvirus 1, Human / genetics*
  • Herpesvirus 1, Human / metabolism
  • Horses
  • Humans
  • Microsomes / metabolism
  • Molecular Sequence Data
  • Protein Biosynthesis
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Sequence Homology, Amino Acid
  • Viral Proteins / biosynthesis
  • Viral Proteins / genetics*

Substances

  • RNA, Messenger
  • UL53 protein, Human herpesvirus 1
  • Viral Proteins

Associated data

  • GENBANK/U34593