Stereospecific dihydroxylation of the styrene vinyl group by purified naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4

J Bacteriol. 1996 Jun;178(11):3353-6. doi: 10.1128/jb.178.11.3353-3356.1996.

Abstract

Naphthalene dioxygenase (NDO) from Pseudomonas sp. strain NCIB 9816-4 adds both atoms of the dioxygen molecule to styrene to form (R)-l-phenyl-1,2-ethanediol. Product formation is tightly coupled to dioxygen consumption and NADH oxidation. NDO oxidizes styrene-d8 at almost the same initial rate as styrene. The results indicate that dioxygen activation by NDO is different from that by cytochrome P-450 and other monooxygenases, which oxidize styrene to styrene 1,2-oxide.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Dioxygenases
  • Hydroxylation
  • Multienzyme Complexes / pharmacology*
  • NAD / metabolism
  • Oxidation-Reduction
  • Oxygenases / pharmacology*
  • Pseudomonas / enzymology*
  • Styrene
  • Styrenes / metabolism*

Substances

  • Multienzyme Complexes
  • Styrenes
  • NAD
  • Styrene
  • Oxygenases
  • Dioxygenases
  • naphthalene dioxygenase