Analysis of suppressor mutations of spoIVCA mutations: occurrence of DNA rearrangement in the absence of site-specific DNA recombinase SpoIVCA in Bacillus subtilis

J Bacteriol. 1996 Jun;178(11):3380-3. doi: 10.1128/jb.178.11.3380-3383.1996.

Abstract

The spoIVCA gene of Bacillus subtilis encodes a site-specific recombinase, which excises a 48-kb skin element from the chromosomal DNA by DNA rearrangement and creates a new composite gene, sigK, on the chromosome. From spoIVCA mutants, we have isolated Spo+ revertants which have no skin element but have an intact sigK gene. This result suggests that the DNA rearrangement can occur in the absence of spoIVCA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics*
  • Base Sequence
  • DNA Nucleotidyltransferases / genetics*
  • Gene Rearrangement*
  • Integrases*
  • Molecular Sequence Data
  • Mutation
  • Recombinases

Substances

  • Recombinases
  • DNA Nucleotidyltransferases
  • Integrases
  • integron integrase IntI1