Evidence for a Beta 1 Integrin Fibronectin Receptor in Candida Tropicalis

J Infect Dis. 1996 Jul;174(1):127-32. doi: 10.1093/infdis/174.1.127.

Abstract

The binding of Candida tropicalis to fibronectin (FN) was studied in order to characterize the FN receptor in this species. FN binding was saturable at a concentration of 1.8 x 10(-9) M and exhibited a Kd of 2.3 x 10(-9) M and a receptor density of 854 receptors per cell. Extracts of C. tropicalis cell membrane at dilutions of 1:100-1:1000 significantly inhibited the binding of 3H-labeled FN to C. tropicalis cells (P < .03). Purified FN, antibodies to the integrin alpha 5 beta 1 (FN receptor on human placenta), and antibodies specific for the integrin beta 1 subunit recognized a C. tropicalis membrane protein of 125 +/- 25 kDa on immunoblots. Immunoprecipitation of radiolabeled proteins from C. tropicalis with purified human FN yielded a protein of 105 +/- 15 kDa. Thus, C. tropicalis expresses a protein with antigenic and functional similarity to the vertebrate beta 1 integrin FN receptor.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Candida / classification
  • Candida / metabolism
  • Candida / ultrastructure*
  • Humans
  • Immunoblotting
  • Membrane Proteins / metabolism
  • Precipitin Tests
  • Receptors, Fibronectin / immunology
  • Receptors, Fibronectin / isolation & purification*

Substances

  • Membrane Proteins
  • Receptors, Fibronectin