Collagen binding of Staphylococcus aureus is a virulence factor in experimental endocarditis

J Infect Dis. 1996 Jul;174(1):83-8. doi: 10.1093/infdis/174.1.83.


The role of Staphylococcus aureus collagen binding in the development of experimental endocarditis was studied. Two isogenic strains of S. aureus, 1 carrying an insertional inactivation of the gene encoding collagen-binding protein, were compared in a rat model of catheter-induced infective endocarditis (i.e.). Separate groups of rats with traumatized aortic valves were intravenously challenged with 1 of the strains. In rats sacrificed 24 h after inoculation, the collagen-binding strain significantly outnumbered the mutant strain (P < .001); however, 1 h after challenge, there was no difference in numbers of the 2 strains. The results were substantiated, using a 1:1 mixture of the parent strain and the mutant as an inoculate. Our findings suggest that collagen binding of S. aureus is important in the sustenance of experimental IE and plays a limited role during the initial attachment of the microorganism to traumatized aortic valves.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Collagen / metabolism*
  • Disease Models, Animal
  • Endocarditis, Bacterial / metabolism*
  • Endocarditis, Bacterial / microbiology*
  • Endocarditis, Bacterial / pathology
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism
  • Female
  • Fibrinogen / metabolism
  • Fibronectins / metabolism
  • Gene Expression Regulation, Bacterial
  • Humans
  • Integrins / genetics*
  • Rats
  • Rats, Wistar
  • Receptors, Collagen
  • Staphylococcal Infections / metabolism*
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / pathogenicity*
  • Time Factors


  • Fibronectins
  • Integrins
  • Receptors, Collagen
  • Fibrinogen
  • Collagen