Characterization of the Saccharomyces cerevisiae FMS1 gene related to Candida albicans corticosteroid-binding protein 1

Curr Genet. 1996 Jul 31;30(2):115-20. doi: 10.1007/s002940050109.


In order to investigate ergosterol metabolism in S. cerevisiae we studied the CM8 mutant strain defective in the regulation of this pathway. A genomic multicopy library was screened to reverse the CM8 phenotype. This allowed us to characterize a new gene, FMS1, which relieves mutant phenotype by extragenic functional complementation. FMS1 may encode a 508 amino-acid protein. The predicted protein shares 35% identity with Cbp1p, a Candida albicans corticosteroid binding-protein. Fms1p also shows a weaker homology with monoamine oxidases. The construction of a FMS1 null-allele yeast strain demonstrated that this gene is not essential for yeast in normal usual laboratory culture conditions. The existence of a gene related to CBP1 of C. albicans in S. cerevisiae strongly suggests a possible function of steroid-binding proteins in yeast general physiology rather than in a process related to pathogenicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Candida albicans / genetics*
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • Fungal Proteins / genetics*
  • Fungicides, Industrial / pharmacology
  • Genes, Fungal
  • Genetic Complementation Test
  • Genetic Vectors
  • Molecular Sequence Data
  • Morpholines / pharmacology
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid


  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • CBF1 protein, S cerevisiae
  • DNA-Binding Proteins
  • FMS1 protein, S cerevisiae
  • Fungal Proteins
  • Fungicides, Industrial
  • Morpholines
  • Saccharomyces cerevisiae Proteins
  • fenpropimorph

Associated data

  • GENBANK/X81848