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, 35 (2), 353-60

A 500-kb Physical Map and Contig From the Harvey ras-1 Gene to the 11p Telomere

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A 500-kb Physical Map and Contig From the Harvey ras-1 Gene to the 11p Telomere

M W Russell et al. Genomics.

Abstract

A contiguous physical map was constructed from the Harvey ras-1 (HRAS1) gene to the 11p telomere. The contig spans approximately 500 kb and is minimally composed of a telomere-containing YAC and P1 and cosmid clones. Included in the contig are 11 sequence-tagged sites derived from P1 and cosmid ends. Three genes were placed on the contig in the following order: telomere-ribonuclease/angiogenin inhibitor (RNH)-Harvey ras-1 (HRAS1)-HRAS1-related complex (HRC). Two novel tetranucleotide repeats (heterozygosity of 66 and 68%) and a complex CA repeat (heterozygosity of 78%) were isolated and characterized.

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