CD40/CD40L interactions and cytokines regulate HIV replication in B cells in vitro

Virology. 1996 Jun 15;220(2):309-19. doi: 10.1006/viro.1996.0319.


Using our in vitro model of normal B cell infection, we investigated whether cellular interactions and/or cytokines directing the B cell response also regulate HIV replication. Phorbol esters and CD40 Ab plus IL4, added prior to infection, substantially increased subsequent viral replication. Postinfection, IL2 with or without IL4 and, to a lesser extent, CD40/CD40L interactions enhanced viral replication. In contrast, IL10 down-regulated HIV replication induced by cytokines, without affecting spontaneous or CD40 Ab-induced replication. Both enhancing and inhibitory effects of cytokines on viral replication were independent of their ability to modulate B cell proliferation. Thus, these two phenomena seem to be independently regulated in human B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / immunology
  • B-Lymphocytes / virology*
  • CD40 Antigens / immunology*
  • CD40 Ligand
  • Cell Division
  • Cells, Cultured
  • Child
  • Cytokines / immunology*
  • Down-Regulation
  • HIV Core Protein p24 / biosynthesis
  • HIV-1 / immunology
  • HIV-1 / physiology*
  • Humans
  • Interleukin-10 / immunology
  • Interleukin-2 / immunology
  • Interleukin-4 / immunology
  • Membrane Glycoproteins / immunology*
  • Sheep
  • T-Lymphocytes / immunology
  • Tumor Necrosis Factor-alpha / metabolism
  • Virus Replication*


  • CD40 Antigens
  • Cytokines
  • HIV Core Protein p24
  • Interleukin-2
  • Membrane Glycoproteins
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • CD40 Ligand
  • Interleukin-4