Enhanced expression of manganese superoxide dismutase mRNA and increased TNFalpha mRNA expression by gastric mucosa in gastric cancer

World J Surg. 1996 Feb;20(2):228-33. doi: 10.1007/s002689900035.

Abstract

Manganese superoxide dismutase (Mn-SOD), a mitochondrial enzyme, is a cytokine-regulated acute-phase protein that protects cells from free radicals. The current investigations examined the in vivo regulation of the expression of Mn-SOD mRNA and tumor necrosis factor alpha (TNFalpha) mRNA in gastric carcinoma tissue. The expression of these transcripts in breast carcinoma tissue also was examined because breast cancer is a much more TNF-sensitive tumor than gastric cancer. TNFalpha mRNA was markedly increased in gastric carcinoma tissue (p < 0.005). There were significantly higher levels of Mn-SOD mRNA in gastric carcinoma tissue than in noncancerous tissue (p < 0.0001). The level of Mn-SOD mRNA in gastric carcinoma tissue was higher than that in breast carcinoma tissue (p < 0.005). Up-regulation of Mn-SOD mRNA in gastric carcinoma tissue most likely serves as a protective mechanism against superoxide radicals and TNF cytotoxicity.

MeSH terms

  • Adult
  • Aged
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism
  • Carcinoma / genetics
  • Carcinoma / metabolism
  • Cytotoxicity, Immunologic / genetics
  • Female
  • Free Radicals
  • Gastric Mucosa / metabolism*
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Male
  • Middle Aged
  • RNA, Messenger / genetics*
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism
  • Superoxide Dismutase / genetics*
  • Superoxides / metabolism
  • Transcription, Genetic
  • Tumor Necrosis Factor-alpha / genetics*
  • Up-Regulation / genetics

Substances

  • Free Radicals
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Superoxides
  • Superoxide Dismutase