A multicopy suppressor gene, MSS10, restores STA2 expression in Saccharomyces cerevisiae strains containing the STA10 repressor gene

Curr Genet. 1996 May;29(6):523-9.


Transcription of the three unlinked, homologous STA1-3 glucoamylase-encoding genes, involved in starch degradation by Saccharomyces cerevisiae, was previously shown to be down-regulated by the presence of STA10, acting via three upstream repression sequence regions that were identified in the STA2 promoter. Here we report the cloning and characterization of a putative transcriptional activator gene, MSS10 (multicopy suppressor of STA10), which, when present in multiple copies, overcomes STA10 repression. Deletion of MSS10, located on chromosome XV, resulted in media-specific extinction of glucoamylase synthesis. The nucleotide sequence of MSS10 is identical to three other genes from S. cerevisiae identified as: FUP1, a gene that enhances iron-limited growth; PHD2, a gene identified for its ability to induce pseudohyphal growth in diploid cells grown on nitrogen-limited media; and MSN1, a gene encoding a transcriptional activator involved in invertase regulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cloning, Molecular
  • DNA, Fungal / genetics
  • Diploidy
  • Gene Expression
  • Genes, Fungal*
  • Genes, Regulator*
  • Genes, Suppressor*
  • Glucan 1,4-alpha-Glucosidase / biosynthesis
  • Glucan 1,4-alpha-Glucosidase / genetics
  • Heterozygote
  • Multigene Family
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Starch / metabolism
  • Transcriptional Activation


  • DNA, Fungal
  • Starch
  • Glucan 1,4-alpha-Glucosidase