Platelet-mediated lymphocyte delivery to high endothelial venules

Science. 1996 Jul 12;273(5272):252-5. doi: 10.1126/science.273.5272.252.

Abstract

Circulating lymphocytes gain access to lymph nodes owing to their ability to initiate rolling along specialized high endothelial venules (HEVs). One mechanism of rolling involves L-selectin binding to peripheral node addressin (PNAd) on HEVs. Activated platelets are shown to bind to circulating lymphocytes and to mediate rolling in HEVs, in vivo, through another molecule, P-selectin, which also interacts with PNAd. In vitro, activated platelets enhanced tethering of lymphocytes to PNAd and sustained lymphocyte rolling, even in the absence of functional L-selectin. Thus, a platelet pathway operating through P-selectin provides a second mechanism for lymphocyte delivery to HEVs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Surface / metabolism*
  • Blood Platelets / physiology*
  • Cell Adhesion
  • Cell Movement
  • Endothelium, Vascular / cytology
  • Humans
  • L-Selectin / physiology
  • Ligands
  • Lymph Nodes / blood supply*
  • Lymph Nodes / cytology
  • Lymphocytes / cytology
  • Lymphocytes / physiology*
  • Membrane Glycoproteins / metabolism
  • Membrane Proteins
  • Mice
  • P-Selectin / metabolism
  • Platelet Activation
  • Receptors, Lymphocyte Homing / metabolism
  • Transfection
  • Tumor Cells, Cultured
  • Venules / cytology

Substances

  • Antigens, Surface
  • L-selectin counter-receptors
  • Ligands
  • Membrane Glycoproteins
  • Membrane Proteins
  • P-Selectin
  • P-selectin ligand protein
  • Receptors, Lymphocyte Homing
  • L-Selectin