Swapping between Fas and granulocyte colony-stimulating factor receptor

J Biol Chem. 1996 Jul 19;271(29):17555-60. doi: 10.1074/jbc.271.29.17555.


Fas belongs to the tumor necrosis factor/nerve growth factor receptor family. The Fas ligand binds to its receptor, Fas, and induces apoptosis in Fas-bearing cells. The granulocyte colony-stimulating factor receptor (G-CSFR) is a member of the hemopoietic growth factor receptor family. G-CSF induces its dimerization and regulates the proliferation and differentiation of neutrophilic granulocytes. We constructed hybrid receptors between Fas and G-CSFR and expressed them in the mouse T cell line WR19L or the mouse myeloid interleukin-3-dependent FDC-P1 cell line. The Fas ligand or an agonistic anti-Fas antibody stimulated proliferation of the FDC-P1 transformants expressing a chimera consisting of the Fas extracellular and G-CSFR cytoplasmic regions. On the other hand, G-CSF could not induce apoptosis in the transformants expressing the chimera consisting of the G-CSFR extracellular and Fas cytoplasmic regions, but these cells were killed by a polyclonal antibody against G-CSFR. These results indicated that receptors belonging to different receptor families can be functionally exchanged and confirm that a homodimer of G-CSFR can transduce the growth signal, whereas Fas must be oligomerized (probably trimerized) to transduce the apoptotic signal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Base Sequence
  • Cell Death
  • Cell Division / drug effects
  • Cell Line
  • DNA Primers
  • Fas Ligand Protein
  • Granulocyte Colony-Stimulating Factor / pharmacology
  • Lymphoma, T-Cell
  • Macromolecular Substances
  • Membrane Glycoproteins / metabolism
  • Membrane Glycoproteins / pharmacology
  • Mice
  • Models, Biological
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Receptors, Granulocyte Colony-Stimulating Factor / biosynthesis
  • Receptors, Granulocyte Colony-Stimulating Factor / isolation & purification
  • Receptors, Granulocyte Colony-Stimulating Factor / physiology*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / metabolism
  • Signal Transduction
  • T-Lymphocytes
  • Transfection
  • fas Receptor / biosynthesis
  • fas Receptor / isolation & purification
  • fas Receptor / physiology*


  • DNA Primers
  • Fas Ligand Protein
  • Fasl protein, mouse
  • Macromolecular Substances
  • Membrane Glycoproteins
  • Receptors, Granulocyte Colony-Stimulating Factor
  • Recombinant Fusion Proteins
  • fas Receptor
  • Granulocyte Colony-Stimulating Factor