Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion

Neuron. 1996 Jun;16(6):1189-96. doi: 10.1016/s0896-6273(00)80145-2.


Depletion of intracellular calcium stores generates a signal that activates Ca2+-permeable channels in the plasma membrane. We have identified a human cDNA, TRPC1A, from a human fetal brain cDNA library. TRPC1A is homologous to the cation channels trp and trpl in Drosophila and is a splice variant of the recently identified cDNA Htrp-1. Expression of TRPC1A in CHO cells induced nonselective cation currents with similar permeabilities for Na+, Ca2+, and Cs+. The currents were activated by intracellular infusion of myo inositol 1,4,5-trisphosphate or thapsigargin. Expression of TRPC1A significantly enhanced increases in the intracellular free calcium concentration induced by Ca2+ restitution after prolonged depletion. Similar results were obtained in Sf9 cells. We conclude that TRPC1A encodes a Ca2+-permeable cation channel activated by depletion of intracellular calcium stores.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Calcium Channels / genetics
  • Calcium Channels / physiology*
  • Cations*
  • Cloning, Molecular
  • Cricetinae
  • Humans
  • Ion Channels / genetics
  • Ion Channels / physiology*
  • Membrane Potentials / physiology
  • Molecular Sequence Data
  • Patch-Clamp Techniques


  • Calcium Channels
  • Cations
  • Ion Channels

Associated data

  • GENBANK/Z73903