The paper reports a re-evaluation of the previous studies on yeast by considering the influence of vacuole upon the dielectric properties of the cell. In this respect, relative permittivity and conductivity of yeast cells dispersed in KCI solutions of various concentrations were measured in the frequency range from 0.1 to 100 MHz. The analysis of data revealed that the beta-dielectric dispersion of yeast cell suspensions is a composite of three (or probably four) distinct sub-dispersions. Since the dielectric response of the cell wall was experimentally avoided (according to Asami et al. (1976) J. Membr. Biol. 28, 169-180), the two-shell model, related to the plasma membrane and the vacuolar membrane, respectively, appeared to be the best approximation for yeast cells. The most relevant parameters obtained with the aid of the two-shell model were as follows. Specific capacitance of the plasma membrane and the vacuolar membrane were 0.703 +/- 0.011 microF/cm2 and 0.483 +/- 0.029 microF/cm2, respectively; electrical conductivity of the cytoplasm and the vacuole interior were 0.515 +/- 0.028 S/m and 3.22 +/- 0.48 S/m; finally, the permittivity of the cytoplasm was 50.6 +/- 2.