Rapid and selective induction of blood eosinophilia in guinea pigs by recombinant human interleukin 5

Cytokine. 1995 Nov;7(8):775-83. doi: 10.1006/cyto.1995.0093.


Interleukin 5 (IL-5) has been implicated as a causal mediator in the development of pulmonary eosinophilia and airway hyperreactivity in human asthma. Supportive evidence for a pathogenic role of IL-5 in this disease has been provided by guinea pig models in which antigen-induced lung eosinophilia and bronchial hyperresponsiveness have been specifically attenuated with a neutralizing antibody to IL-5. In the present report, we describe a rapid mechanism-based model of IL-5-induced eosinophilia in the guinea pig. Our results show that intravenous injection of human IL-5 induced a 5-10-fold increase in the percentage and number of eosinophils in blood within 1 hour. In contrast, neutrophils and mononuclear cells were not recruited during this time. The increase in eosinophils was blocked by pretreatment of animals with an anti-IL5 monoclonal antibody (TRFK5) in doses similar to those which inhibit eosinophilia in guinea pig asthma models. Furthermore, dexamethasone, a potent inhibitor of eosinophilia in guinea pig asthma, profoundly suppressed the eosinophilia induced by human IL-5. This rapid model will be useful for elucidating the eosinophil activating properties of IL-5 in vivo and may potentially facilitate the development of IL-5 receptor antagonists for the specific blockade of the eosinophilic inflammation observed in human asthma.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Antibodies, Monoclonal / pharmacology*
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Eosinophilia / chemically induced*
  • Eosinophilia / immunology
  • Eosinophilia / prevention & control
  • Guinea Pigs
  • Humans
  • Immunoglobulin G / analysis
  • Injections, Intravenous
  • Interleukin-5* / administration & dosage
  • Interleukin-5* / immunology
  • Kinetics
  • Male
  • Rats
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / immunology
  • Time Factors


  • Antibodies, Monoclonal
  • Immunoglobulin G
  • Interleukin-5
  • Recombinant Proteins