Multiresidue liquid chromatographic method for simultaneous determination of pyrethroid insecticides in fruits and vegetables

J AOAC Int. 1995 Nov-Dec;78(6):1474-80.

Abstract

A simple and rapid liquid chromatographic (LC) method has been developed for simultaneous determination of 9 pyrethroid insecticides (biphenthrin, cypermethrin, fenpropathrin, fenvalerate, flucythrinate, methothrin, permethrin, py-115, and tetramethrin) in fruits and vegetables. Residues are extracted from crops with methanol and partitioned with toluene. Extracts are cleaned up by Florisil-charcoal column chromatography. LC separation is performed on a muBondapak C18 stainless steel column with acetonitrile-deionized water as mobile phase. The insecticides are detected at 206 nm with 0.03 absorbance unit full scale. Recoveries of 9 pyrethroid insecticides from 6 crops (cucumbers, tomatoes, cabbages, apples, pears, and peaches) fortified at 0.5-5.0 mg/kg were 62.7-129.2%. Detection limits were about 0.05 mg/kg, except for py-115, for which detection limit was 0.10 mg/kg.

MeSH terms

  • Chromatography, Liquid / methods*
  • Food Analysis
  • Food Contamination*
  • Fruit / chemistry
  • Insecticides / analysis*
  • Pesticide Residues / analysis*
  • Pyrethrins / analysis*
  • Vegetables / chemistry

Substances

  • Insecticides
  • Pesticide Residues
  • Pyrethrins