Abstract
We report the isolation and characterisation of cDNAs encoding three different, human members of the cysteine-rich secretory protein (CRISP) family. The novel CRISP-1 exists in five cDNA subtypes differing by the presence or absence of a stretch coding for a C-terminal cysteine-rich domain so far found in all members of the family, and by the length of their 3'-untranslated region. CRISP-2 cDNA corresponds to the previously described TPX1 form, with so far unreported 5'-untranslated sequence heterogeneities while CRISP-3 cDNA codes for a new, unique protein. Northern blot analysis of various human organs indicates that CRISP-1 transcripts are epididymis-specific whereas CRISP-2/TPX1 transcripts are detected mainly in the testis and also in the epididymis. CRISP-3 transcripts are more widely distributed and found predominantly in the salivary gland, pancreas and prostate, and in less abundance in the epididymis, ovary, thymus and colon. A protein reacting with an anti-mouse CRISP-1 antibody was isolated from human epididymal extracts and N-terminal sequencing revealed that it corresponded to the CRISP-1 cDNA we have isolated. In contrast to findings on its rat counterpart epididymal protein DE/acidic epididymal glycoprotein (AEG), no significant association of CRISP-1 with human spermatozoa was observed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Adhesion Molecules
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DNA, Complementary
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Enzyme-Linked Immunosorbent Assay
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Epididymis / metabolism*
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Gene Library
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Glycoproteins / analysis
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Glycoproteins / biosynthesis*
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Glycoproteins / chemistry*
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Humans
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Male
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Membrane Glycoproteins*
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Membrane Proteins
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Mice
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Organ Specificity
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Polymerase Chain Reaction
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Rats
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Recombinant Proteins / analysis
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Salivary Proteins and Peptides / analysis
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Salivary Proteins and Peptides / biosynthesis*
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Salivary Proteins and Peptides / chemistry*
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Seminal Plasma Proteins*
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Spermatozoa / metabolism
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Testis / metabolism*
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Transcription, Genetic
Substances
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CRISP1 protein, human
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CRISP2 protein, human
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CRISP3 protein, human
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Cell Adhesion Molecules
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Crisp1 protein, mouse
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Crisp2 protein, mouse
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Crisp3 protein, mouse
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Crisp3 protein, rat
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DNA, Complementary
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Glycoproteins
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Membrane Glycoproteins
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Membrane Proteins
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Oligodeoxyribonucleotides
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RNA, Messenger
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Recombinant Proteins
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Salivary Proteins and Peptides
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Seminal Plasma Proteins
Associated data
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GENBANK/X95237
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GENBANK/X95238
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GENBANK/X95239
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GENBANK/X95240