The gene encoding mouse intestinal trefoil factor: structural organization, partial sequence analysis and mapping to murine chromosome 17q

Gene. 1996 Jun 1;171(2):249-53. doi: 10.1016/0378-1119(96)00074-1.


Trefoil peptides, a growing family of secretory molecules, have been identified mainly in the gastrointestinal tract of humans, rodents and amphibians. In the present study, the nucleotide sequence of a large portion (81%) of the gene encoding murine intestinal trefoil factor (mITF) and its whole genomic organization were determined. The mITF gene contains three exons distributed over 5 kb of genomic DNA. The genomic sequence is highly conserved, as compared with that of the rat and human ITF, and contains several AP-1-binding sites, the consensus binding site for the transcription factor Sp1, and a sequence homologous to a heat-shock element. Fluorescence in situ hybridization was used to assign ITF to chromosome 17 of the murine genome, a region syntenic with the trefoil gene cluster on human chromosome 21q22.3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Chromosome Mapping
  • Cloning, Molecular
  • Conserved Sequence
  • Growth Substances / chemistry
  • Growth Substances / genetics*
  • Growth Substances / metabolism
  • Heat-Shock Proteins / chemistry
  • Heat-Shock Proteins / genetics
  • Intestinal Mucosa / metabolism
  • Mice / genetics*
  • Molecular Sequence Data
  • Mucins*
  • Muscle Proteins*
  • Neuropeptides*
  • Peptides / chemistry
  • Peptides / genetics*
  • Peptides / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Transcription Factor AP-1 / metabolism
  • Transcription Factors / metabolism
  • Trefoil Factor-2
  • Trefoil Factor-3


  • Growth Substances
  • Heat-Shock Proteins
  • Mucins
  • Muscle Proteins
  • Neuropeptides
  • Peptides
  • TFF3 protein, rat
  • Transcription Factor AP-1
  • Transcription Factors
  • Trefoil Factor-2
  • Trefoil Factor-3

Associated data

  • GENBANK/U46858