Cloning, sequencing and functional studies of the gene encoding human GTP cyclohydrolase I

Gene. 1996 Jun 1;171(2):285-90. doi: 10.1016/0378-1119(95)00886-1.

Abstract

We have identified a genomic clone containing the 5' regulatory region of the gene GTP-CH encoding human GTP cyclohydrolase I. The transcription start point (tsp) was mapped by 5'-rapid amplification of cDNA ends (5'-RACE). The 2.6-kb region upstream from the tsp showed promoter activity when ligated upstream from a reporter gene. The truncation of approximately 2 kb of the promoter did not change expression activity, while a further removal of 243 bp halved the activity. The promoter contains CCAAT and TATA boxes. The GC-rich region close to the tsp, which contains several putative Sp1-responsive elements, is required for maximum promoter activity. Interferon-gamma treatment of B-cells transfected with reporter constructs had no influence on the expression activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Line / drug effects
  • Cloning, Molecular
  • Drosophila melanogaster / enzymology
  • Drosophila melanogaster / genetics
  • GTP Cyclohydrolase / genetics*
  • GTP Cyclohydrolase / metabolism
  • Genes, Reporter
  • Humans
  • Interferon-gamma / pharmacology
  • Molecular Sequence Data
  • Regulatory Sequences, Nucleic Acid
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Transfection

Substances

  • Interferon-gamma
  • GTP Cyclohydrolase

Associated data

  • GENBANK/L29478