Vectorette PCR isolation of microsatellite repeat sequences using anchored dinucleotide repeat primers

N J Lench; A Norris; A Bailey; A Booth; A F Markham

doi:10.1093/nar/24.11.2190

Vectorette PCR isolation of microsatellite repeat sequences using anchored dinucleotide repeat primers

Nucleic Acids Res. 1996 Jun 1;24(11):2190-1. doi: 10.1093/nar/24.11.2190.

Authors

N J Lench¹, A Norris, A Bailey, A Booth, A F Markham

Affiliation

¹ Molecular Medicine Unit, Clinical Sciences Building, St James's University Hospital, University of Leeds, UK.

Abstract

We have developed a vectorette PCR approach to provide an improved method for isolation of microsatellite repeats. The modified procedure relies on PCR amplification using a vectorette-specific primer in combination with one of a panel of anchored dinucleotide repeat primers. The target DNA to be screened for microsatellite sequences can be from YAC, P1, cosmid, bacteriophage or plasmid clones. We have used this technique to isolate novel, polymorphic microsatellite repeats from clones containing the amelogenin gene (AMGX) located on human chromosome Xp22.3.

MeSH terms

Amelogenin
Base Sequence
DNA / chemistry
DNA / isolation & purification*
DNA Primers*
Dental Enamel Proteins / genetics*
Dinucleotide Repeats
Genetic Vectors*
Humans
Microsatellite Repeats*
Molecular Sequence Data
Polymerase Chain Reaction*
X Chromosome

Substances

Amelogenin
DNA Primers
Dental Enamel Proteins
DNA