Differential Requirements for alpha4 Integrins During Fetal and Adult Hematopoiesis

Cell. 1996 Jun 28;85(7):997-1008. doi: 10.1016/s0092-8674(00)81301-x.

Abstract

Mice chimeric for the expression of alpha4 integrins were used to dissect the roles of these receptors in development and traffic of lymphoid and myeloid cells. During fetal life, T cell development is alpha4 independent, but after birth further production of T cells becomes alpha4 dependent. Precursors for both T and B cells require alpha4 integrins for normal development within the bone marrow. In contrast, monocytes and natural killer cells can develop normally without alpha4 integrins. Thus, there are lymphocyte-specific, developmentally regulated requirements for alpha4 integrins in hematopoiesis in the bone marrow. We also show that alpha4 integrins are essential for T cell homing to Peyer's patches, but not to other secondary lymphoid organs, including spleen, lymph nodes, and intestinal epithelium.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Age Factors
  • Animals
  • Antigens, CD / genetics
  • Antigens, CD / immunology*
  • B-Lymphocytes / chemistry
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • Cell Differentiation / immunology
  • Cell Movement / immunology*
  • Chimera
  • Fetus / physiology
  • Flow Cytometry
  • Gene Expression / immunology
  • Hematopoiesis / immunology*
  • Integrin alpha4
  • Integrins / genetics
  • Integrins / immunology*
  • Killer Cells, Natural / chemistry
  • Killer Cells, Natural / cytology
  • Killer Cells, Natural / immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Monocytes / chemistry
  • Monocytes / cytology
  • Monocytes / immunology
  • Mutation / immunology
  • Peyer's Patches / cytology
  • Polymerase Chain Reaction
  • Stem Cells / chemistry
  • Stem Cells / cytology
  • Stem Cells / immunology
  • T-Lymphocytes / chemistry
  • T-Lymphocytes / cytology*
  • T-Lymphocytes / immunology

Substances

  • Antigens, CD
  • Integrins
  • Integrin alpha4