Antibodies to phospholipid-binding plasma proteins and occurrence of thrombosis in patients with systemic lupus erythematosus

Clin Immunol Immunopathol. 1996 Jul;80(1):16-22. doi: 10.1006/clin.1996.0089.


There is accumulating evidence that anti-phospholipid (aPL) antibodies in the sera of patients with autoimmune diseases bind to a complex of anionic phospholipids and plasma phospholipid-binding proteins, namely beta 2-glycoprotein I (beta 2-GPI) and prothrombin. It has been suggested that a conformational change in beta 2-GPI, induced by binding either to anionic phospholipids or to the oxygen molecules on the irradiated microtiter plate, reveals cryptic antigenic epitope(s) in the native protein. We used an enzyme-linked immunoassay for measuring antibodies against two phospholipid-binding proteins, i.e., beta 2-GPI and prothrombin, absorbed to an irradiated plate in an unselected series of 139 patients with systemic lupus erythematosus (SLE). Elevated levels of antibodies against beta 2-GPI were found in 49% of patients and antibodies against prothrombin in 34% of patients. Both antibodies were significantly associated with deep venous thrombosis in patients with SLE (P = 0.009 for both antibodies). Accordingly, testing of these antibodies seems to be clinically useful in evaluating the risk of thrombosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antibodies, Anticardiolipin / blood
  • Antibodies, Antiphospholipid / blood*
  • Apolipoproteins / immunology
  • Female
  • Glycoproteins / immunology
  • Humans
  • Lupus Coagulation Inhibitor / blood
  • Lupus Erythematosus, Systemic / blood
  • Lupus Erythematosus, Systemic / complications
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Middle Aged
  • Prothrombin / immunology
  • Thrombosis / blood
  • Thrombosis / etiology
  • Thrombosis / immunology*
  • beta 2-Glycoprotein I


  • Antibodies, Anticardiolipin
  • Antibodies, Antiphospholipid
  • Apolipoproteins
  • Glycoproteins
  • Lupus Coagulation Inhibitor
  • beta 2-Glycoprotein I
  • Prothrombin