The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein

FEBS Lett. 1996 Jun 3;387(2-3):127-31. doi: 10.1016/0014-5793(96)00478-4.

Abstract

The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of the eukaryotic 14-3-3 protein family based on three lines of evidence. First, the sequence of an eight amino acid tryptic peptide, obtained from immunopurified IP, matched that of a highly conserved region of the 14-3-3 proteins. Second, an authentic member of the 14-3-3 family, recombinant Arabidopsis GF14omega, caused inactivation of phospho-NR in a magnesium-dependent manner identical to IP. Third, an anti-GF14 monoclonal antibody cross-reacted with IP and anti-IP monoclonal antibodies cross-reacted with GF14omega.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 14-3-3 Proteins
  • Amino Acid Sequence
  • Animals
  • Arabidopsis Proteins*
  • Calcium-Binding Proteins / pharmacology
  • Female
  • Immunoblotting
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Nitrate Reductase (NADH)
  • Nitrate Reductases / antagonists & inhibitors*
  • Phosphorylation
  • Plant Leaves / enzymology
  • Plant Proteins / chemistry
  • Plant Proteins / isolation & purification
  • Plant Proteins / pharmacology*
  • Precipitin Tests
  • Proteins / chemistry
  • Proteins / isolation & purification
  • Proteins / pharmacology*
  • Recombinant Proteins / pharmacology
  • Spinacia oleracea / enzymology*
  • Tyrosine 3-Monooxygenase*

Substances

  • 14-3-3 Proteins
  • Arabidopsis Proteins
  • Calcium-Binding Proteins
  • GF14 protein, Arabidopsis
  • Plant Proteins
  • Proteins
  • Recombinant Proteins
  • Tyrosine 3-Monooxygenase
  • Nitrate Reductases
  • Nitrate Reductase (NADH)