Complementation of an Arabidopsis thaliana biotin auxotroph with an Escherichia coli biotin biosynthetic gene

Mol Gen Genet. 1996 Jun 12;251(3):261-6. doi: 10.1007/BF02172516.


The Arabidopsis thaliana biotin auxotroph biol was rendered prototrophic by transformation with a chimeric transgene containing the Escherichia coli bio A gene driven by a constitutive promoter. The bio A gene encodes the biotin biosynthetic enzyme 7, 8-diaminopelargonic acid aminotransferase. Unlike the untransformed control plants, transgenic plants expressing the bacterial transgene synthesized biotin and grew to maturity without biotin-deficiency symptoms. These findings demonstrate that bio1/bio1 mutant plants are defective in the gene encoding 7,8-diaminopelargonic acid aminotransferase.

MeSH terms

  • Amino Acids, Diamino / genetics*
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Base Sequence
  • Biotin / biosynthesis*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Gene Expression
  • Genes, Bacterial / genetics
  • Genes, Plant / genetics
  • Molecular Sequence Data
  • Mutation*
  • Plants, Genetically Modified / genetics
  • RNA, Messenger / analysis
  • RNA, Plant / analysis
  • Transgenes / genetics


  • Amino Acids, Diamino
  • RNA, Messenger
  • RNA, Plant
  • 7,8-diaminopelargonic acid
  • Biotin