Abstract
Stem cell factor (SCF) interacts with the receptor tyrosine kinase c-Kit and has potent effects on hematopoiesis. We have examined the role of JAK2 in the SCF signal transduction pathway. JAK2 and c-Kit were constitutively associated, and treatment with SCF resulted in rapid and transient tyrosine phosphorylation of JAK2. Incubation of cells with JAK2 antisense oligonucleotides resulted in significant decreases in SCF-induced proliferation. These data suggest that JAK2 plays a role in SCF-induced proliferation.
MeSH terms
-
Animals
-
Base Sequence
-
Blotting, Western
-
Cell Division / drug effects
-
Cell Line
-
DNA, Antisense
-
Electrophoresis, Polyacrylamide Gel
-
Humans
-
Janus Kinase 2
-
Mice
-
Molecular Sequence Data
-
Phosphoproteins / analysis
-
Phosphorylation / drug effects
-
Phosphotyrosine / metabolism
-
Protein-Tyrosine Kinases / metabolism*
-
Proto-Oncogene Proteins c-kit / metabolism*
-
Proto-Oncogene Proteins*
-
Signal Transduction*
-
Stem Cell Factor / pharmacology*
-
Tumor Cells, Cultured
Substances
-
DNA, Antisense
-
Phosphoproteins
-
Proto-Oncogene Proteins
-
Stem Cell Factor
-
Phosphotyrosine
-
Protein-Tyrosine Kinases
-
Proto-Oncogene Proteins c-kit
-
JAK2 protein, human
-
Jak2 protein, mouse
-
Janus Kinase 2