JAK2 is constitutively associated with c-Kit and is phosphorylated in response to stem cell factor

Acta Haematol. 1996;95(3-4):224-8. doi: 10.1159/000203882.

Abstract

Stem cell factor (SCF) interacts with the receptor tyrosine kinase c-Kit and has potent effects on hematopoiesis. We have examined the role of JAK2 in the SCF signal transduction pathway. JAK2 and c-Kit were constitutively associated, and treatment with SCF resulted in rapid and transient tyrosine phosphorylation of JAK2. Incubation of cells with JAK2 antisense oligonucleotides resulted in significant decreases in SCF-induced proliferation. These data suggest that JAK2 plays a role in SCF-induced proliferation.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Western
  • Cell Division / drug effects
  • Cell Line
  • DNA, Antisense
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Janus Kinase 2
  • Mice
  • Molecular Sequence Data
  • Phosphoproteins / analysis
  • Phosphorylation / drug effects
  • Phosphotyrosine / metabolism
  • Protein-Tyrosine Kinases / metabolism*
  • Proto-Oncogene Proteins c-kit / metabolism*
  • Proto-Oncogene Proteins*
  • Signal Transduction*
  • Stem Cell Factor / pharmacology*
  • Tumor Cells, Cultured

Substances

  • DNA, Antisense
  • Phosphoproteins
  • Proto-Oncogene Proteins
  • Stem Cell Factor
  • Phosphotyrosine
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins c-kit
  • JAK2 protein, human
  • Jak2 protein, mouse
  • Janus Kinase 2