Characterization of Toronto virus capsid protein expressed in baculovirus

Arch Virol. 1996;141(5):865-75. doi: 10.1007/BF01718161.


Toronto virus (TV), previously called "minireovirus", a human calicivirus classified as genogroup 2 and phylogenetic type P2-A, was originally described in association with diarrhea in children. The second open reading frame, encoding the capsid protein of TV24, was expressed in a baculovirus recombinant. The recombinant baculovirus produced a protein (rTV) with an apparent molecular mass of 58 kDa that self-assembled into virus-like particles approximately 30 nm in diameter with a density of 1.29 g/ml. Antigenic and immunogenic characteristics of these particles were determined by protein immunoblot, immunoprecipitation, and enzyme immunoassay. Seroconversion to the rTV protein was detected in 6 of 8 (75%) patients from a recent outbreak of gastroenteritis associated with a virus of similar phylogenetic type. These results confirm and extend the previous reports of the expression of the Norwalk and Mexico virus capsid proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Baculoviridae / genetics
  • Base Sequence
  • Caliciviridae / chemistry*
  • Capsid / biosynthesis*
  • Capsid / immunology
  • Capsid / isolation & purification
  • Molecular Sequence Data
  • Rabbits
  • Recombinant Proteins / biosynthesis
  • Spodoptera
  • Virus Assembly


  • Recombinant Proteins